A high throughput methodology for susceptibility testing of Mycobacterium tuberculosis isolates
Tashmin Rampersad1, Mantha Makume1, Parveen Sobia1
1Department of Infection Prevention and Control, Nelson R Mandela School of Medicine, School of Laboratory of Medicine and Medical Science, College of Health Science, University of KwaZulu-Natal, 719 Umbilo Road, Durban 4001, South Africa.
Abstract:
MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi-point inoculation. The results were compared with classic agar dilution and the MTT assay. The multi-point inoculation method was reproducible with all drugs and correlated with classic agar dilution and MTT assay. This methodology can be used for routine breakpoint drug susceptibility testing (DST) and for MIC determination.
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