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Related Experiment Videos

Surface Plasmodium sugar-binding components evidenced by fluorescent neoglycoproteins.

J Schrevel, M Philippe, F Bernard

    Biology of the Cell
    |January 1, 1986
    PubMed
    Summary

    Researchers identified a lectin-like protein on the surface of Plasmodium parasites. This sugar-binding component, crucial for parasite invasion, interacts with N-acetylglucosamine and other sugars.

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    Area of Science:

    • Parasitology
    • Molecular Biology
    • Biochemistry

    Background:

    • The surface of intraerythrocytic malaria parasites (Plasmodium) possesses molecules involved in host cell invasion.
    • Understanding these surface components is critical for developing anti-malarial strategies.

    Purpose of the Study:

    • To identify and characterize sugar-binding components on the surface of endoerythrocytic Plasmodium berghei and Plasmodium chabaudi.
    • To define the binding specificity and localization of these surface molecules.

    Main Methods:

    • Utilized a panel of neoglycoproteins to probe for sugar-binding activity.
    • Employed fluorescence microscopy and flow cytofluorometry for detection.
    • Assessed the protein nature of the binding component using trypsin treatment.

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    Main Results:

    • A sugar-binding component was detected on the surface of endoerythrocytic stages and merozoites of Plasmodium berghei and P. chabaudi.
    • The binding capacity was lost after trypsin treatment, confirming its protein nature.
    • This lectin-like protein exhibited primary binding to N-acetylglucosamine and secondary binding to alpha-D-glucose, beta-D-galactose, and alpha-L-fucose.

    Conclusions:

    • The study suggests the presence of at least one type of lectin-like protein on the Plasmodium parasite surface.
    • This protein possesses an extended binding site capable of accommodating multiple sugar units.
    • The findings define the localization of this lectin-like protein to the parasite surface.