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Differentially expressed Secretoglobin 1C1 gene in nasal polyposis
1Department of Bioengineering, Faculty of Engineering, Adana Science and Technology University, Adana, Turkey.
Cellular and Molecular Biology (Noisy-Le-Grand, France)
|February 8, 2018
Summary
Secretoglobin 1C1 gene promoter polymorphisms are linked to increased Secretoglobin 1C1 expression in nasal polyps (NP). These genetic variations may contribute to NP susceptibility in the Turkish population.
Area of Science:
- Genetics
- Immunology
- Otorhinolaryngology
Background:
- Nasal polyps (NP) are characterized by chronic nasal mucosa inflammation.
- The pathophysiology of NP involves a balance of pro-inflammatory and anti-inflammatory cytokines.
- Secretoglobin 1C1 (SCGB1C1) gene, involved in synthesizing odor molecule binding proteins (OBPs) and regulating cytokines, may play a role in NP.
Purpose of the Study:
- To investigate the effect of Secretoglobin 1C1 gene promoter polymorphisms on SCGB1C1 gene expression in patients with nasal polyps.
- To determine the association between SCGB1C1 promoter variations and the development of NP.
Main Methods:
- Sequencing of the Secretoglobin 1C1 gene promoter in 48 subjects (24 NP patients, 22 controls).
- Quantification of Secretoglobin 1C1 gene expression in nasal mucosa using RT-PCR.
- Analysis of the relationship between identified polymorphisms and gene expression levels.
Main Results:
- Secretoglobin 1C1 expression was significantly elevated in nasal polyps compared to controls (P=0.003).
- Three polymorphisms were identified in the SCGB1C1 promoter, with rs113795008 and rs2280540 being more frequent in NP patients (P=0.005, P=0.045).
- The rs113795008 (G/G) genotype correlated with higher SCGB1C1 mRNA expression in NP (P=0.009).
Conclusions:
- A correlation exists between elevated Secretoglobin 1C1 expression and its promoter polymorphisms in nasal polyps.
- These promoter variations may contribute to NP susceptibility, particularly in the Turkish population.
- Alterations in SCGB1C1 gene promoter function can impact gene expression relevant to NP pathogenesis.
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