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Ultrasensitive microfluidic paper-based electrochemical/visual biosensor based on spherical-like cerium dioxide

Xiaolu Sun1, He Wang1, Yannan Jian1

  • 1Institute for Advanced Interdisciplinary Research, University of Jinan, Jinan 250022, PR China.

Biosensors & Bioelectronics
|February 8, 2018
PubMed
Summary

This study presents a novel electrochemical biosensor for sensitive microRNA detection using gold nanorods and cerium dioxide. The device offers a low detection limit for accurate microRNA quantification.

Keywords:
Cerium dioxideElectrochemical biosensorMicroRNAMicrofluidic paper-based analytical device

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Area of Science:

  • Analytical Chemistry
  • Biosensors
  • Nanotechnology

Background:

  • MicroRNA (miRNA) detection is crucial for disease diagnostics.
  • Existing methods often require complex procedures and expensive equipment.
  • Development of sensitive and accessible miRNA detection platforms is needed.

Purpose of the Study:

  • To develop a sensitive electrochemical biosensor for microRNA detection.
  • To utilize microfluidic paper-based analytical devices (μPADs) for enhanced analysis.
  • To employ cerium dioxide - gold@glucose oxidase (CeO2-Au@GOx) as an electrochemical probe for signal amplification.

Main Methods:

  • Synthesized gold nanorods (Au NRs) on μPADs for improved conductivity.
  • Modified hairpin probes via gold-sulfur bonds.
  • Utilized GOx to catalyze glucose, producing H2O2, which was electrocatalyzed by CeO2.
  • Performed electrochemical and visual detection methods.

Main Results:

  • Achieved a wide linear range (1.0 fM to 1000 fM) with a low detection limit (0.434 fM) via electrochemical measurement.
  • Visual detection showed a linear range of 10 fM to 1000 fM with a detection limit of 7.382 fM.
  • Demonstrated significant electrochemical signal from H2O2 reduction.

Conclusions:

  • The developed electrochemical biosensor demonstrates high sensitivity and a low detection limit for miRNA.
  • The platform shows potential for practical applications in miRNA detection.
  • Integration of Au NRs and CeO2-Au@GOx probe enhances biosensor performance on μPADs.