Related Experiment Video
Updated: Feb 14, 2026

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
Published on: April 15, 2022
Biodegradable, pH-sensitive chitosan beads obtained under microwave radiation for advanced cell culture
Marek Piątkowski1, Łukasz Janus1, Julia Radwan-Pragłowska1
1Department of Biotechnology and Physical Chemistry, Cracow University of Technology, Cracow, Poland.
Abstract:
A new type of promising chitosan beads with advanced properties were obtained under microwave radiation according to Green Chemistry principles. Biomaterials were prepared using chitosan as raw material and glutamic acid/1,5-pentanodiol mixture as crosslinking agents. Additionally beads were modified with Tilia platyphyllos extract to enhance their antioxidant properties. Beads were investigated over their chemical structure by FT-IR analysis. Also their morphology has been investigated by SEM method. Additionally swelling capacity of the obtained hydrogels was determined. Lack of cytotoxicity has been confirmed by MTT assay. Proliferation studies were carried out on L929 mouse fibroblasts. Advanced properties of the obtained beads were investigated by studying pH sensitivity and antioxidant properties by DPPH method. Also susceptibility to degradation and biodegradation by Sturm Test method was evaluated. Results shows that proposed chitosan beads and their eco-friendly synthesis method can be applied in cell therapy and tissue engineering.
Related Concept Videos
Biological Effects of Radiation
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Urine Studies II: Urine Culture and Sensitivity Test
Radiation: Applications
The average...
Absorption of Radiation
Radiation Pressure: Problem Solving
The average value of the rate of momentum transfer divided by the absorbing area represents the average force...

