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Updated: Feb 14, 2026
06:01
Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
Published on: February 2, 2019
7.9K
Functionally coated polyethersulfone hollow fiber membranes: A substrate for enhanced HepG2/C3A functions
Surendra Kumar Verma1, Akshay Modi1, Atul Kumar Singh2
1Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India.
Colloids and Surfaces. B, Biointerfaces
|February 8, 2018
Summary
This study developed novel gelatin-coated hollow fiber membranes (HFMs) for bio-artificial liver (BAL) support systems. These membranes enhance liver cell attachment and function, offering a promising bridge to transplantation.
Area of Science:
- Biomaterials Engineering
- Regenerative Medicine
- Hepatology
Background:
- Hollow fiber membranes (HFMs) are crucial for bio-artificial liver (BAL) support systems, acting as a temporary measure for patients awaiting liver transplantation or regeneration.
- A significant challenge in BAL systems is achieving adequate liver cell attachment and sustained function on HFM surfaces.
Purpose of the Study:
- To develop and characterize novel glutaraldehyde-crosslinked gelatin-coated polyethersulfone HFMs (X-gel-PT HFMs).
- To evaluate the suitability of X-gel-PT HFMs for hepatocyte culture and BAL support systems by assessing cell attachment, proliferation, and metabolic activity.
Main Methods:
- Fabrication of X-gel-PT HFMs using a dry-wet spinning method with a triple orifice spinneret.
- Comprehensive characterization of HFMs including morphology, surface properties, mechanical strength, thermal stability, and pore structure.
- In vitro assessment of hemocompatibility, HepG2/C3A cell attachment, proliferation, urea synthesis, and albumin secretion using microscopy and biochemical assays.
Main Results:
- Characterization confirmed successful GTA-crosslinked gel-coating, creating an extracellular matrix-like environment.
- In vitro hemocompatibility tests indicated suitability for blood-contact applications.
- X-gel-PT HFMs demonstrated significantly enhanced HepG2/C3A cell attachment and proliferation.
- Improved functional and metabolic activity of HepG2/C3A cells was evidenced by high urea synthesis and albumin secretion.
Conclusions:
- The developed X-gel-PT HFMs provide a suitable substrate for hepatocyte culture and mass culture.
- These novel HFMs show significant potential for the development of advanced bio-artificial liver support systems.
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