Selected landscape phage probe as selective recognition interface for sensitive total prostate-specific antigen...

Area of Science:

• Biotechnology
• Biosensor Development
• Cancer Biomarker Detection

Background:

• Total prostate-specific antigen (t-PSA) is a key biomarker for prostate cancer diagnosis.
• Traditional antibody-based detection methods can have limitations in sensitivity and cost.
• Phage display technology offers a novel approach for generating specific binding agents.

Purpose of the Study:

• To select and characterize phage probes specific for t-PSA.
• To develop and evaluate a biosensor utilizing these phage probes for t-PSA detection.
• To compare the performance of phage-based biosensors with conventional methods like ELISA.

Main Methods:

• Biopanning of a phage display library to isolate t-PSA-specific phage clones.
• Characterization of phage binding affinity and specificity using phage capture assays.
• Development of sandwich ELISA and differential pulse voltammetry (DPV) assay systems incorporating the phage probes.

Main Results:

• A specific phage clone displaying the fusion peptide ATRSANGM showed high affinity for t-PSA.
• Both ELISA and DPV assays demonstrated high specificity and reliability for serum sample analysis.
• The DPV method exhibited a wider linear range, lower limit of detection (3 pg/mL), and better agreement with authoritative methods compared to ELISA.

Conclusions:

• Landscape phage displaying specific peptides can serve as effective capture probes in biosensors.
• The phage-probe-based DPV immunosensor offers highly sensitive t-PSA detection at the picogram per milliliter level.
• Phage-based biosensors present a promising alternative to antibody-based methods for cancer biomarker detection due to their stability and homogeneity.