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A partial fraction is a component of a rational expression represented as the sum of simpler fractions. When a rational function is expressed as a ratio of two polynomials, it can often be decomposed into a sum of fractions whose denominators are simpler polynomials, typically linear or irreducible quadratic factors. This process is called partial fraction decomposition, and it is used to simplify complex expressions for integration, solving equations, or analysis.Partial fraction decomposition...
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Partially hydrolyzed guar gum as a potential prebiotic source.

Deepak Mudgil1, Sheweta Barak1, Ami Patel2

  • 1Department of Dairy & Food Technology, Mansinhbhai Institute of Dairy & Food Technology, Mehsana 384002, India.

International Journal of Biological Macromolecules
|February 8, 2018
PubMed
Summary

Partially hydrolyzed guar gum effectively supports the growth of probiotic Lactic Acid Bacteria strains. This research indicates its potential as a prebiotic to enhance gut microflora and beneficial bacteria populations.

Keywords:
Dietary fiberGuar gumLactic acid bacteriaPrebioticViscosity

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Area of Science:

  • Microbiology
  • Food Science
  • Biochemistry

Background:

  • Guar galactomannan can be enzymatically hydrolyzed to create partially hydrolyzed guar gum (PHGG).
  • PHGG has potential applications as a prebiotic, a substance that selectively stimulates the growth of beneficial gut bacteria.
  • Understanding the prebiotic potential of PHGG requires studying its effect on probiotic growth.

Purpose of the Study:

  • To investigate the growth-promoting effects of partially hydrolyzed guar gum (PHGG) on specific probiotic strains.
  • To compare the efficacy of PHGG as a prebiotic source against glucose and native guar gum.
  • To assess the assimilation of PHGG by Lactic Acid Bacteria (LAB) strains.

Main Methods:

  • Enzymatic hydrolysis of guar galactomannan to produce PHGG.
  • Cultivation of six probiotic strains (Lactic Acid Bacteria) with glucose, PHGG, and native guar gum.
  • Measurement of bacterial growth using optical density and viable cell counts.
  • Monitoring of pH changes in the growth medium.
  • API 50 CH test to determine galactose and mannose assimilation capabilities.

Main Results:

  • All six probiotic strains demonstrated the ability to assimilate PHGG.
  • Significant increases in optical density and viable cell counts were observed in the presence of PHGG.
  • A concomitant decrease in medium pH indicated bacterial metabolic activity.
  • Streptococcus thermophilus MD2 showed the highest growth (7.78 log CFU/ml), while P. parvulus AI1 exhibited lower growth (7.24 log CFU/ml).
  • Lactobacillus and Weissella strains also showed varying degrees of growth with PHGG.

Conclusions:

  • Partially hydrolyzed guar gum (PHGG) serves as a viable prebiotic compound.
  • PHGG effectively supports the growth of tested probiotic Lactic Acid Bacteria strains.
  • PHGG holds potential for stimulating the growth of beneficial gut microflora and probiotic bacteria.