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Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Published on: July 6, 2012
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Radiation-induced alternative transcripts as detected in total and polysome-bound mRNA
Amy Wahba1, Michael C Ryan2, Uma T Shankavaram1
1Radiation Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USA.
Oncotarget
|February 9, 2018
Summary
Ionizing radiation alters gene expression by inducing alternative splicing and selectively associating mRNA isoforms with polysomes, impacting cellular radioresponse.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Alternative splicing is crucial for posttranscriptional gene regulation.
- Understanding radiation's impact on gene expression is vital for cancer therapy.
Purpose of the Study:
- To investigate how ionizing radiation affects alternative splicing in glioblastoma stem-like cells.
- To compare radiation-induced changes in the transcriptome and translatome.
Main Methods:
- RNA-sequencing (RNA-Seq) was performed on control and irradiated human glioblastoma stem-like cells (NSC11).
- SpliceSeq software was used to identify radiation-induced splice variants in total mRNA (transcriptome) and polysome-bound mRNA (translatome).
Main Results:
- Radiation induced 92 splice events in the transcriptome and 280 in the translatome.
- Only 24 splice events overlapped between the radiation-induced transcriptome and translatome.
- Radiation selectively associated mRNA isoforms with polysomes, influencing translational control.
Conclusions:
- Ionizing radiation induces alternative splicing and affects mRNA isoform polysome association.
- Existing alternative transcripts may play a role in radiation-induced translational control and cellular radioresponse.
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