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Updated: Feb 14, 2026
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
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Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM
Lina Mörén1, Richard Perryman2, Tim Crook3
1Department of Chemistry, Umeå University, SE 901 87, Umeå, Sweden.
BMC Cancer
|February 10, 2018
Summary
Glioblastoma subtypes with defective arginine synthesis (ASS1 deficient) show distinct metabolic profiles compared to ASS1 positive tumors. These metabolic differences may offer new diagnostic and therapeutic strategies for glioblastoma multiforme (GBM).
Area of Science:
- Biochemistry
- Metabolomics
- Cancer Biology
Background:
- Glioblastomas (GBM) exhibit varying arginine metabolism due to argininosuccinate synthetase (ASS1) gene silencing.
- ASS1-deficient GBM are arginine auxotrophic and sensitive to arginine degradation, correlating with a poorer prognosis.
- Metabolic profiling can identify diagnostic markers and therapeutic targets in GBM subtypes.
Purpose of the Study:
- To investigate metabolic differences between ASS1-deficient and ASS1-positive GBM.
- To explore the potential for non-invasive detection of GBM subtypes.
- To identify novel therapeutic opportunities based on metabolic profiles.
Main Methods:
- Metabolomic analysis using gas chromatography-time-of-flight mass spectrometry (GC-TOFMS) and liquid chromatography-time-of-flight mass spectrometry (LC-TOFMS).
- Targeted analysis of 29 amino acids.
- Bioinformatic evaluation using orthogonal partial least squares-discriminant analysis (OPLS-DA) and pathway analysis.
Main Results:
- Significant metabolic differences were observed between ASS1-deficient and ASS1-positive GBM.
- ASS1-positive cells showed elevated metabolites, including those in the arginine biosynthetic pathway.
- ASS1-deficient cells displayed altered arginine, citrulline, and general amino acid metabolism, with notable perturbations upon ADI-PEG20 treatment.
Conclusions:
- Distinct metabolomes exist between ASS1-negative and ASS1-positive GBM.
- These metabolic differences hold potential for diagnostic and therapeutic applications in GBM.
- Further research is warranted to explore the clinical utility of these findings.
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