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Crystallization of Recombinant α-Actinin and Related Proteins
Karina Persson1, Lars Backman2
1Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden.
Abstract:
When it comes to crystallization each protein is unique. It can never be predicted beforehand in which condition the particular protein will crystallize or even if it is possible to crystallize. Still, by following some simple checkpoints the chances of obtaining crystals are increased. The primary checkpoints are purity, stability, concentration, and homogeneity. High-quality protein crystals are needed. This protocol will allow an investigator to: clone, express, and crystallize a protein of interest.
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