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Updated: Feb 14, 2026

Dosimetry for Cell Irradiation using Orthovoltage 40-300 kV X-Ray Facilities
Published on: February 20, 2021
Screening of biomarkers for liver adenoma in low-dose-rate γ-ray-irradiated mice
Takashi Sugihara1, Satoshi Tanaka1, Ignacia Braga-Tanaka1
1a Department of Radiobiology , Institute for Environmental Sciences , Rokkasho Kamikita , Aomori , Japan.
Purpose:
Chronic low-dose-rate (20 mGy/day) γ-irradiation increases the incidence of hepatocellular adenomas (HCA) in female B6C3F1 mice. The purpose of this study is to identify potential serum biomarkers for these HCAs by a new approach.
Material And Methods:
Microarray analysis were performed to compare the gene expression profiles of HCAs from mice exposed to low-dose-rate γ-rays with those of normal livers from non-irradiated mice. From the differentially expressed genes, those for possibly secretory proteins were selected. Then, the levels of the proteins in sera were analysed by ELISA.
Results:
Microarray analysis identified 4181 genes differentially expressed in HCAs (>2.0-fold). From these genes, those for α-fetoprotein (Afp), α-1B-glycoprotein (A1bg) and serine peptidase inhibitor Kazal type-3 (Spink3) were selected as the genes for candidate proteins. ELISA revealed that the levels of Afp and A1bg proteins in sera significantly increased and decreased, respectively, in low-dose-rate irradiated mice with HCAs and also same tendency was observed in human patients with hepatocellular carcinomas.
Conclusion:
These results indicate that A1bg could be a new serum biomarker for liver tumor. This new approach of using microarray to select genes for secretory proteins is useful for prediction of novel tumor markers in sera.
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