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Updated: Feb 14, 2026
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
Published on: March 4, 2016
Micrografting chronic lower extremity ulcers with mechanically disaggregated skin using a micrograft preparation
Rosario Miranda1, Eleonora Farina2, Michele A Farina3
1Medical Angiologist, Specialista Ambulatoriale Branca di Angiologia - Azienda Sanitaria Locale NA3-Sud Distretto di Nola (NA), Italy.
Objective:
The Rigenera system is a new standardised micrograft preparation system. It works by means of automated mechanical disaggregation of small tissue samples, extracting only the smallest cells (<50µm). The aim of this study was to retrospectively evaluate patients affected by chronic ulcers and who were treated with the micrograft preparation method.
Method:
Chronic ulcers have been included regardless of the cause. The specimen was collected with a 3mm diameter biopsy punch and immediately dissociated by means of the Rigenera System. The obtained suspension was placed on a scaffold of equine collagen.
Results:
We included 15 patients (four males, 11 females) with a mean age of 72.2±8.41 (mean±standard deviation) years. In seven patients the ulcers were related to the complications of diabetes, post-traumatic in a further three diabetic patients, vasculitis in one patient, and four patients had venous leg ulcers (VLUs). The median main diameter was 5.0cm and the median estimated area was 43.96cm2. The ulcers were present from a mean of 4.50±2.30 months before inclusion in this study. At the second week the wounds were reduced by 37.33%±19.35%, at the week eight, nine patients (60.0%) were healed, and at week 16, 13 (86.7%) were healed. The quality of scars was good and did not deteriorate at the six month follow-up.
Conclusion:
The simplicity of the approach, the minimal invasiveness of the specimen collection, and the good quality of scarring of healed wounds, confirmed in the follow-up, makes this micrograft preparation method a useful tool to use on large or complex wounds.
Insights
The Rigenera system effectively treats chronic ulcers using micrografts. This minimally invasive method achieved an 86.7% healing rate by week 16, with good scar quality.
Area of Science:
- Regenerative medicine
- Wound healing
- Tissue engineering
Background:
- Chronic ulcers represent a significant clinical challenge.
- Current treatments can be invasive and have limited efficacy.
- Novel approaches for wound regeneration are needed.
Purpose of the Study:
- To evaluate the efficacy of the Rigenera micrograft preparation system.
- To assess patient outcomes for chronic ulcers treated with this method.
Main Methods:
- Utilized the Rigenera system for automated mechanical disaggregation of tissue samples.
- Collected micrografts (<50µm) and applied them to an equine collagen scaffold.
- Retrospectively analyzed data from 15 patients with chronic ulcers.
Main Results:
- Significant wound size reduction observed by week 2 (37.33%).
- High healing rates achieved: 60% by week 8 and 86.7% by week 16.
- Good scar quality was noted, with no deterioration at 6-month follow-up.
Conclusions:
- The Rigenera system offers a simple and minimally invasive method for micrograft preparation.
- This approach is a valuable tool for treating large or complex chronic wounds.
- Positive outcomes in healing rates and scar quality support its clinical utility.
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