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Autophagy suppresses the proliferation of renal carcinoma cell

Z-L Wang1, Q Deng, T Chong

  • 1Department of Urology, Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China. zhenlongw2001@163.com.

European Review for Medical and Pharmacological Sciences
|February 10, 2018
PubMed
Abstract

Insights

Autophagy suppresses renal cell carcinoma (RCC) growth. Enhancing autophagy may offer a new therapeutic strategy for advanced RCC, impacting tumor proliferation in vitro and in vivo.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Medicine

Background:

  • Renal cell carcinoma (RCC) is a significant health concern.
  • Autophagy plays a complex role in cancer, requiring further investigation in RCC.

Purpose of the Study:

  • To investigate the role of autophagy in the proliferation of renal cell carcinoma (RCC).
  • To explore autophagy as a potential therapeutic target for advanced RCC.

Main Methods:

  • Established RCC cell lines with altered autophagy-related protein 7 (Atg7) expression (overexpression and knockdown).
  • Assessed cell proliferation using MTT assays and protein expression via Western blot.
  • Utilized xenograft mouse models to evaluate autophagy's in vivo effect on RCC growth.

Main Results:

  • Atg7 overexpression increased LC3II levels, indicating enhanced autophagy.
  • Atg7 knockdown reduced LC3II levels, suggesting inhibited autophagy.
  • Atg7 overexpression suppressed RCC cell growth, while Atg7 knockdown accelerated tumor growth in vivo.

Conclusions:

  • Autophagy inhibits the growth of RCC cells both in vitro and in vivo.
  • Autophagy represents a promising new therapeutic target for advanced renal cell carcinoma.

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