Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Composition of Body Fluids01:29

Composition of Body Fluids

2.7K
Water functions as a solvent accommodating various solutes, which can be categorized under electrolytes and non-electrolytes. Non-electrolytes are usually held together by covalent bonds, restricting them from dissociating in solution, thereby leading to a lack of electrically charged components upon dissolving in water. They are predominantly organic molecules, such as glucose, creatinine, and urea. Electrolytes, on the other hand, are compounds that can break down into ions in water.
2.7K
Tracheostomy Suctioning II: Procedure01:23

Tracheostomy Suctioning II: Procedure

2.0K
Tracheostomy suctioning is a vital nursing procedure that involves removing secretions from the tracheostomy tube to maintain airway patency and prevent respiratory complications. Nurses need to understand the proper technique for tracheostomy suctioning to ensure patient safety and comfort. In this guide, we will outline the step-by-step process for performing tracheostomy suctioning, including preparing the sterile field, donning personal protective equipment (PPE), lubricating and connecting...
2.0K
Suctioning the Nasopharyngeal Airway01:29

Suctioning the Nasopharyngeal Airway

3.7K
Nasopharyngeal suctioning is a procedure to remove secretions from the upper part of the respiratory tract that the patient cannot clear independently. It helps maintain airway patency and prevents complications such as aspiration pneumonia.
Equipment Required
3.7K
Suctioning the Oropharyngeal Airway01:25

Suctioning the Oropharyngeal Airway

1.0K
In preparing for oropharyngeal airway suctioning, a nurse must gather all necessary equipment, including a suction unit with tubing, a prepackaged suction kit, sterile gloves, water or saline for irrigation, a water-soluble lubricant, and additional personal protective equipment (such as a gown, mask, and goggles) to control infections.
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...
1.0K
Classifying Matter by Composition03:35

Classifying Matter by Composition

91.8K
Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures. 
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated. 
A mixture is composed of two or...
91.8K
Tracheostomy Suctioning I: Pre-Procedural Steps01:26

Tracheostomy Suctioning I: Pre-Procedural Steps

2.5K
Tracheostomy suctioning is a critical procedure healthcare professionals perform to maintain a patent airway in patients with a tracheostomy tube. This procedure is necessary when secretions accumulate in the airway, causing respiratory distress. Here is a step-wise procedural guide for performing tracheostomy suctioning using an open system.
Equipment Required
First, gather all necessary equipment: a sterile suction catheter, a sterile disposable container, sterile gloves, a towel or...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vision for Exposomics-Scale Investigation: A Heuristic for Exposure-Wide Association Studies (ExWAS).

Environmental science & technology·2026
Same author

Exposomics Crosses the Rubicon: Let the Building Begin.

Environmental science & technology·2026
Same author

Inviting ecosystems into the exposome framework.

Exposome·2026
Same author

The Exposome journal on hiatus: not even a flesh wound.

Exposome·2026
Same author

Lysophosphatidylcholines are associated with amyloidosis in early stages of Alzheimer's disease.

Nature aging·2025
Same author

Leveraging open cheminformatics tools for non-targeted metabolomics analysis of C. elegans: a workflow comparison and application to strains related to xenobiotic metabolism and neurodegeneration.

Analytical and bioanalytical chemistry·2025

Related Experiment Video

Updated: Feb 14, 2026

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

12.6K

Human Suction Blister Fluid Composition Determined Using High-Resolution Metabolomics.

Megan M Niedzwiecki1, Pradnya Samant2, Douglas I Walker3

  • 1Department of Environmental Health, Rollins School of Public Health , Emory University , Atlanta , Georgia 30322 , United States.

Analytical Chemistry
|February 10, 2018
PubMed
Summary
This summary is machine-generated.

Interstitial fluid (ISF), analyzed via suction blister fluid (SBF), reveals a rich metabolome valuable for health diagnostics. This study highlights SBF as a promising source for identifying novel metabolite biomarkers.

More Related Videos

Granulocyte-dependent Autoantibody-induced Skin Blistering
12:23

Granulocyte-dependent Autoantibody-induced Skin Blistering

Published on: October 12, 2012

10.9K
Author Spotlight: Multi-Layered Approach to Understand Postnatal Functions of Pancreatic Islets in Non-Human Primates
03:39

Author Spotlight: Multi-Layered Approach to Understand Postnatal Functions of Pancreatic Islets in Non-Human Primates

Published on: November 8, 2024

818

Related Experiment Videos

Last Updated: Feb 14, 2026

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

12.6K
Granulocyte-dependent Autoantibody-induced Skin Blistering
12:23

Granulocyte-dependent Autoantibody-induced Skin Blistering

Published on: October 12, 2012

10.9K
Author Spotlight: Multi-Layered Approach to Understand Postnatal Functions of Pancreatic Islets in Non-Human Primates
03:39

Author Spotlight: Multi-Layered Approach to Understand Postnatal Functions of Pancreatic Islets in Non-Human Primates

Published on: November 8, 2024

818

Area of Science:

  • Biochemistry
  • Human Physiology
  • Analytical Chemistry

Background:

  • Interstitial fluid (ISF) is crucial for cellular function and contains biomarkers relevant to health.
  • ISF's molecular similarity to plasma suggests its diagnostic potential, but comprehensive analysis is lacking.
  • Suction blister fluid (SBF) serves as a surrogate for ISF, offering a non-invasive sampling method.

Purpose of the Study:

  • To comprehensively analyze the metabolome of ISF using SBF.
  • To compare the metabolite composition of SBF with plasma.
  • To identify potential metabolite biomarkers in ISF for diagnostics and health monitoring.

Main Methods:

  • Collected SBF from 10 human volunteers.
  • Utilized untargeted high-resolution metabolomics (HRM) for metabolite profiling.
  • Analyzed a broad spectrum of metabolites including amino acids, lipids, and nucleotides.

Main Results:

  • Detected a wide array of metabolites in SBF, including exogenous compounds.
  • Identified unique and elevated systemic and skin-derived metabolite biomarkers in SBF.
  • Demonstrated significant correlations between many metabolites in SBF and plasma.

Conclusions:

  • Untargeted HRM profiling of SBF provides valuable insights into human health.
  • SBF is a promising biofluid for discovering and monitoring health-relevant metabolites.
  • This study establishes SBF as a viable source for comprehensive metabolomic analysis.