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IL8 and IL16 levels indicate serum and plasma quality
Olga Kofanova1, Estelle Henry1, Rocio Aguilar Quesada2
1Integrated Biobank of Luxembourg, Strassen, Luxembourg.
Clinical Chemistry and Laboratory Medicine
|February 10, 2018
Summary
Extended pre-centrifugation delays impact serum and plasma sample quality. Interleukin-16 (IL16) and Interleukin-8 (IL8) concentrations can serve as reliable markers to identify samples with processing delays.
Area of Science:
- Clinical chemistry
- Biomarker discovery
- Sample integrity
Background:
- Pre-centrifugation time significantly affects the quality of serum and plasma samples.
- Identifying reliable markers for sample processing delays is crucial for accurate diagnostic results.
Purpose of the Study:
- To identify and validate biomarkers that indicate extended pre-centrifugation delays in blood samples.
- To assess the diagnostic performance of these biomarkers in qualifying sample quality.
Main Methods:
- Screened twenty cytokines in serum, EDTA plasma, and citrate plasma samples for concentration changes due to pre-centrifugation delays at room temperature.
- Validated the two cytokines exhibiting the most significant changes using R&D Systems ELISA kits.
Main Results:
- Elevated IL16 concentrations (>313 pg/mL in EDTA plasma, >897 pg/mL in EDTA plasma after 48h) and IL8 concentrations (>125 pg/mL in serum, >21.5 pg/mL in citrate plasma, >528 pg/mL in serum after 48h) indicated extended pre-centrifugation delays.
- Specific concentration thresholds were established for IL16 and IL8 to identify samples with delays exceeding 24 and 48 hours.
Conclusions:
- Simple, commercially available ELISA assays for IL16 and IL8 can accurately identify serum and plasma samples with undocumented pre-analytical delays.
- These assays provide robust tools for qualifying legacy sample collections, ensuring data reliability.
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