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Is oxygen availability a limiting factor for in vitro folliculogenesis?
Riccardo Talevi1, Sam Sudhakaran2, Vincenza Barbato1
1Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy.
Plos One
|February 10, 2018
Summary
Optimizing oxygen levels during in vitro culture of ovarian tissue strips is crucial. Controlled oxygen availability enhances follicle quality, viability, and development, improving fertility preservation methods.
Area of Science:
- Reproductive Biology
- Biomedical Engineering
- Cell Culture Technology
Background:
- Ovarian tissue transplantation for fertility preservation in cancer patients carries a risk of tumor cell reintroduction.
- Complete in vitro folliculogenesis is a promising alternative but is hindered by incomplete understanding of the in vivo microenvironment.
- Oxygen availability is a critical, yet understudied, factor influencing ovarian follicle development in vitro.
Purpose of the Study:
- To investigate the impact of oxygen tension on ovarian follicle health and growth during in vitro culture.
- To develop a predictive model for dissolved oxygen concentration within cultured ovarian tissue.
- To optimize in vitro culture conditions for improved follicle survival and development.
Main Methods:
- Ovarian cortical strips from bovine (animal model) and human tissues were cultured in conventional (CD) and gas permeable (PD) dishes.
- Culture conditions varied in media column height and gaseous oxygen tension over 3, 6, and 9 days.
- Follicle quality, primordial to primary and primary to secondary stage progression, and viability (live-dead assay) were assessed.
Main Results:
- Gas permeable dishes with optimized medium height and oxygen tension significantly improved follicle quality and viability in bovine ovarian strips.
- These optimized conditions, when applied to human ovarian strip cultures, also resulted in enhanced follicle quality, viability, and developmental progression.
- A predictive model for dissolved oxygen concentration in ovarian tissue under various culture conditions was developed.
Conclusions:
- Modulating oxygen availability is a key factor in maintaining ovarian follicle health and promoting survival and development during in vitro culture.
- Optimized oxygen conditions can improve the yield of healthy secondary follicles for potential future oocyte maturation.
- This research provides crucial insights for advancing in vitro folliculogenesis techniques for fertility preservation.
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