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Updated: Jan 15, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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Custom-Made Oocytes to Clone Non-human Primates.

Jose B Cibelli1, John B Gurdon2

  • 1Departments of Animal Science and Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.

Cell
|February 10, 2018
PubMed
Summary
This summary is machine-generated.

Researchers successfully cloned two macaque monkeys using fetal fibroblasts. This breakthrough in somatic cell nuclear transfer (SCNT) was achieved by improving epigenetic modifications in egg cells.

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Primate research

Background:

  • Somatic cell nuclear transfer (SCNT) is a technique for cloning mammals.
  • SCNT efficiency in primates remains low due to early developmental roadblocks.
  • Epigenetic reprogramming is crucial for successful SCNT.

Purpose of the Study:

  • To establish a more efficient SCNT method for primates.
  • To overcome epigenetic barriers in early SCNT development.
  • To produce healthy cloned primates.

Main Methods:

  • Utilized fetal fibroblasts as donor cells.
  • Artificially enhanced epigenetic modifiers in the oocyte.
  • Performed somatic cell nuclear transfer in macaque monkeys.

Main Results:

  • Successfully produced two healthy cloned macaque monkeys.
  • Demonstrated the viability of the enhanced SCNT technique.
  • Overcame critical early developmental roadblocks in SCNT.

Conclusions:

  • Enhanced epigenetic modification in oocytes significantly improves SCNT success rates in primates.
  • This study presents a viable method for cloning primates.
  • The findings pave the way for further advancements in primate reproductive technologies.