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miR-145 overexpression triggers alteration of the whole transcriptome and inhibits breast cancer development

Peng Ye1, Yu Shi1, Nairui An1

  • 1Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|February 10, 2018
PubMed

Insights

MicroRNA-145 (miR-145) inhibits breast cancer progression by affecting cell proliferation, migration, and invasion. This study identifies miR-145 targets and potential biomarkers within the competing endogenous RNA network.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play a role in cancer development.
  • miR-145 is recognized as an anti-oncomiRNA.
  • The precise mechanisms of miR-145 in breast cancer remain incompletely understood.

Purpose of the Study:

  • To elucidate the comprehensive mechanisms of miR-145 in breast cancer.
  • To investigate the impact of miR-145 overexpression on breast cancer cell functions.
  • To identify novel molecular targets and biomarkers associated with miR-145.

Main Methods:

  • Next-generation sequencing for transcriptome profiling.
  • Cellular function assays (proliferation, colony formation, migration, invasion) following miR-145 overexpression.
  • Analysis of the competing endogenous RNA (ceRNA) network.

Main Results:

  • miR-145 overexpression significantly inhibited breast cancer cell proliferation, colony formation, migration, and invasion.
  • Transcriptome analysis revealed widespread alterations induced by miR-145.
  • A list of 49 target messenger RNAs (mRNAs) and specific non-coding RNAs regulated by miR-145 was identified.
  • Evidence suggests miR-145 regulates the ceRNA network in breast cancer.

Conclusions:

  • miR-145 functions as a tumor suppressor in breast cancer.
  • miR-145 influences breast cancer progression through transcriptome modulation and ceRNA network regulation.
  • The identified miR-145 targets and non-coding RNAs represent potential biomarkers for breast cancer diagnosis and prognosis.
  • This research provides a foundation for further investigation into miR-145 and ceRNA networks in breast cancer.
Keywords:
Breast cancerTranscriptomeceRNAcircRNAlncRNAmRNAmiR-145miRNA

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