An interferometric imaging biosensor using weighted spectrum analysis to confirm DNA monolayer films with attogram...

Area of Science:

• Biosensing
• Nanotechnology
• Molecular Diagnostics

Background:

• Interferometric imaging biosensors are used for DNA monolayer film detection on silicon microarrays.
• Current limitations include low accuracy and sensitivity for low DNA content and point mutation detection.

Purpose of the Study:

• To develop an enhanced interferometric imaging biosensor with weighted spectrum analysis for improved DNA monolayer film confirmation.
• To increase the accuracy and sensitivity of DNA detection on microarrays.

Main Methods:

• Utilized weighted spectrum analysis to extract and reconstruct interferometric signals from DNA molecules.
• Integrated specific DNA ligation for single nucleotide mismatch detection.

Main Results:

• Reduced relative error in thickness detection from 88.94% to 4.15%.
• Achieved mass sensitivity of 20 attograms (ag) per unit area.
• Demonstrated high selectivity and accuracy in lung cancer mutation detection.

Conclusions:

• The proposed biosensor significantly improves DNA detection accuracy and sensitivity.
• It offers reliable quantitative detection for DNA microarrays, outperforming FRET in measurement veracity.
• The biosensor is capable of detecting single nucleotide mismatches and has potential in clinical diagnostics.