Related Experiment Videos
The proliferative T-lymphocyte response to streptokinase
Journal of Clinical & Laboratory Immunology
|June 1, 1986
Summary
Purified streptokinase activates T-lymphocytes in previously sensitized adults, but not newborns. This immune response to streptokinase was inhibited by several common drugs.
Area of Science:
- Immunology
- Microbiology
Background:
- Streptokinase is an enzyme produced by Streptococcus pyogenes.
- T-lymphocytes play a crucial role in cell-mediated immunity.
Purpose of the Study:
- To investigate the T-lymphocyte response to purified streptokinase.
- To determine if the response is due to prior sensitization.
Main Methods:
- In vitro proliferation assays using T-lymphocytes from healthy adults and newborns.
- Dose-response and kinetic studies.
- Testing the inhibitory effects of methylprednisolone, cyclosporin A, theophyllamine, and verapamil.
Main Results:
- Purified streptokinase induced a proliferative T-lymphocyte response in 40% of healthy adults tested.
- No response was observed in T-lymphocytes from newborns, suggesting prior in vivo sensitization.
- Methylprednisolone, cyclosporin A, theophyllamine, and verapamil inhibited the response in a dose-dependent manner.
Conclusions:
- The proliferative T-lymphocyte response to streptokinase is mediated by T-lymphocytes from individuals previously sensitized in vivo.
- This immune response can be modulated by immunosuppressive and other pharmacological agents.