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Updated: Feb 14, 2026
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Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
Published on: January 25, 2021
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miR-148a-3p promotes rabbit preadipocyte differentiation by targeting PTEN.
Hongbing He1, Mingcheng Cai1, Jiaoyue Zhu2
1Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Sichuan, Chengdu, 611130, China.
In Vitro Cellular & Developmental Biology. Animal
|February 11, 2018
Summary
MicroRNAs (miRNAs) play a key role in fat cell development. This study shows miR-148a-3p promotes adipogenesis by targeting PTEN, highlighting miRNA
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Emerging data suggest microRNAs (miRNAs) are crucial in adipogenesis.
- Detailed mechanisms regulating fat cell differentiation remain largely unknown.
Purpose of the Study:
- To investigate the role and mechanism of miR-148a-3p in rabbit adipogenesis.
- To identify the target gene of miR-148a-3p involved in preadipocyte differentiation.
Main Methods:
- Quantitative PCR (qPCR) and western blotting to analyze gene and protein expression.
- In vitro differentiation of rabbit preadipocytes.
- Luciferase reporter assay to validate miRNA-target interaction.
- siRNA-mediated knockdown of PTEN.
Main Results:
- miR-148a-3p levels increased during rabbit white adipose tissue growth and preadipocyte differentiation.
- Overexpression of miR-148a-3p upregulated key adipogenic markers (PPARγ, C/EBPα, FABP4) and promoted triglyceride accumulation.
- Downregulation of miR-148a-3p inhibited preadipocyte differentiation.
- miR-148a-3p directly targets PTEN, and PTEN inhibition promotes adipogenesis.
Conclusions:
- miR-148a-3p promotes rabbit preadipocyte differentiation by inhibiting PTEN expression.
- This study elucidates a novel miRNA-mediated regulatory pathway in adipogenesis.
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