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Updated: Jun 19, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
The Madness of Microbiome: Attempting To Find Consensus "Best Practice" for 16S Microbiome Studies
Jolinda Pollock1,2, Laura Glendinning2, Trong Wisedchanwet2
1Animal and Veterinary Sciences, Scotland's Rural College (SRUC), Edinburgh, United Kingdom jolinda.pollock@sruc.ac.uk.
Abstract:
The development and continuous improvement of high-throughput sequencing platforms have stimulated interest in the study of complex microbial communities. Currently, the most popular sequencing approach to study microbial community composition and dynamics is targeted 16S rRNA gene metabarcoding. To prepare samples for sequencing, there are a variety of processing steps, each with the potential to introduce bias at the data analysis stage. In this short review, key information from the literature pertaining to each processing step is described, and consequently, general recommendations for future 16S rRNA gene metabarcoding experiments are made.
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