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Updated: Feb 14, 2026

Screening Cotton Genotypes for Reniform Nematode Resistance
Published on: May 2, 2019
Validating a probe from GhSERK1 gene for selection of cotton genotypes with somatic embryogenic capacity
Taiza da Cunha Soares1, Carliane Rebeca Coelho da Silva1, Julita Maria Frota Chagas Carvalho2
1Post-Graduation in Biotechnology, Renorbio/ Federal Rural University of Pernambuco, Rua Manoel de Medeiros, s/n - Dois Irmãos, 52171-900, Recife, Pernambuco, Brazil.
Abstract:
Substantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures. High expression of transcripts was found in embryogenic genotypes, and the results were confirmed by the RT-PCR-blotting using a non-radioactive probe. The regeneration ability was confirmed in embryogenic genotypes. We confirmed that GhSERK1 can be used as marker for estimating the somatic embryogenesis ability of cotton plants.
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