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Updated: Feb 14, 2026

Biology of Microbial Communities - Interview
Published on: May 28, 2007
Influence of reflux ratio on two-stage anoxic/oxic with MBR for leachate treatment: Performance and microbial
Jianbo Liu1, Zhiyong Tian2, Panyue Zhang3
1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Abstract:
A lab-scale two-stage Anoxic/Oxic with MBR (AO/AO-MBR) system was operated for 81 days for leachate treatment with different reflux ratio (R). The best system performances were observed with a R value of 150%, and the average removal efficiencies of chemical oxygen demand, ammonia and total nitrogen were 85.6%, 99.1%, and 77.6%, respectively. The microbial community were monitored and evaluated using high-throughput sequencing. Proteobacteria were dominant in all process. Phylogenetic trees were described at species level, genus Thiopseudomonas, Amaricoccus, Nitrosomonas and Nitrobacter played significant roles in nitrogen removal. Co-occurrence analyzing top 20 genera showed that Nitrosomonas-Nitrobacter presented perfect positive relationship, as well as Paracoccus-Brevundimonas and Pusillimonas-Halobacteriovorax.
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