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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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PSMB8 regulates glioma cell migration, proliferation, and apoptosis through modulating ERK1/2 and PI3K/AKT signaling

Bing-Ya Yang1, Jing-Wei Song2, Hong-Zhi Sun2

  • 1Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, 211166, China; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu, 211166, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|February 12, 2018
PubMed

Insights

Researchers identified PSMB8 as a potential therapeutic target for glioma. Inhibiting PSMB8 suppressed glioma cell migration, proliferation, and tumor growth, suggesting its role in glioma progression.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Glioma is an aggressive brain tumor requiring novel therapeutic strategies.
  • Understanding glioma pathogenesis is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of PSMB8 in glioma.
  • To evaluate PSMB8 as a potential therapeutic target for glioma treatment.

Main Methods:

  • Western blot and immunohistochemistry to assess PSMB8 and Ki-67 expression.
  • In vitro assays (wound-healing, trans-well, CCK-8, flow cytometry, colony formation) to study cell migration and proliferation.
  • In vivo xenograft models to validate findings and assess tumor growth.
  • Analysis of signaling pathways (ERK1/2, PI3k/AKT).

Main Results:

  • PSMB8 expression was elevated in glioma tissues.
  • PSMB8 inhibition reduced glioma cell migration and proliferation by altering key protein expressions (cyclins, cadherins, vimentin).
  • PSMB8 downregulation induced glioma cell apoptosis via caspase-3 upregulation.
  • In vivo studies confirmed that PSMB8 suppression inhibited tumor growth.
  • ERK1/2 and PI3k/AKT pathways were implicated in PSMB8-mediated effects.

Conclusions:

  • PSMB8 is significantly associated with glioma cell migration, proliferation, and apoptosis.
  • Targeting PSMB8 demonstrates therapeutic potential for glioma.
  • PSMB8 may serve as a novel prognostic biomarker for glioma patients.
Keywords:
AKTERK1/2GliomaPSMB8

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