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Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
Published on: December 12, 2015
A stimuli-responsive fluorescence platform for simultaneous determination of d-isoascorbic acid and Tartaric acid
Yanmei Zhao1, Haiyan Yuan1, Xinling Zhang1
1Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservoir, Wanzhou, Chongqing, 404100, P.R. China; School of Environment and Chemical Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing, 404100, P.R. China.
Abstract:
An activatable fluorescence monitoring platform based on a novel Maillard reaction product from d-glucose and L-arginine was prepared through a facile one-pot approach and applied for simultaneous detection of d-isoascorbic acid and tartaric acid. In this work, the new Maillard reaction product GLA was first obtained, and its fluorescence intensity can be effectively quenched by KMnO4, resulting from a new complex (GLA-KMnO4) formation between GLA and KMnO4. Upon addition of d-isoascorbic acid or tartaric acid, an enhanced fluorescence was observed under the optimumed experimental conditions, indicating a stimuli-responsive fluorescence turn on platform for d-isoascorbic acid or tartaric acid can be developed. The corresponding experimental results showed that this turn on fluorescence sensing platform has a high sensitivity for d-isoascorbic acid or tartaric acid, because the detection limits were 5.9μM and 21.5μM, respectively. Additionally, this proposed sensing platform was applied to simultaneously detection of d-isoascorbic acid and tartaric acid in real tap water samples with satisfactory results.
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