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Updated: Feb 14, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
In Situ Construction of Protein-Based Semisynthetic Biosensors
Tsuyoshi Ueda1, Tomonori Tamura1, Itaru Hamachi1,2
1Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering , Kyoto University , Katsura, Nishikyo-ku, Kyoto 615-8510 , Japan.
Abstract:
Chemically constructed biosensors consisting of a protein scaffold and an artificial small molecule have recently been recognized as attractive analytical tools for the specific detection and real-time monitoring of various biological substances or events in cells. Conventionally, such semisynthetic biosensors have been prepared in test tubes and then introduced into cells using invasive methods. With the impressive advances seen in bioorthogonal protein conjugation methodologies, however, it is now becoming feasible to directly construct semisynthetic biosensors in living cells, providing unprecedented tools for life-science research. We discuss here recent efforts regarding the in situ construction of protein-based semisynthetic biosensors and highlight their uses in the visualization and quantification of biomolecules and events in multimolecular and crowded cellular systems.
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