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Related Experiment Video

Updated: Feb 14, 2026

Molecular Evolution of the Tre Recombinase
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Switch and Trace: Recombinase Genetics in Zebrafish.

Tom J Carney1, Christian Mosimann2

  • 1Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology, and Research (A*STAR), Singapore.

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|February 13, 2018
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Summary
This summary is machine-generated.

Recombinase-based genetic tools offer dynamic control over transgenes in zebrafish, enabling advanced applications like gene expression regulation and lineage tracing. These methods enhance the study of development and disease modeling in this versatile model organism.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Transgenic technologies are crucial for in vivo cell and genetic manipulation.
  • Traditionally, transgenes were static, limiting their functional versatility.
  • DNA recombinase systems have revolutionized transgene control.

Purpose of the Study:

  • To review advancements in recombinase-based genetic techniques in zebrafish.
  • To discuss the application of these tools in studying development, homeostasis, and disease.
  • To explore future directions for recombinase genetics in zebrafish.

Main Methods:

  • Utilizing recombinase systems like Cre/lox, Flp/FRT, and ΦC31 for transgene manipulation.
  • Integrating these systems with zebrafish transgenesis and genome editing technologies.
  • Leveraging zebrafish's imaging capabilities for genetic studies.

Main Results:

  • Recombinase-controlled transgenes allow for regulated gene expression, conditional mutagenesis, and genetic lineage tracing.
  • These methods provide versatile control over transgene activity and function.
  • The combination of recombinase genetics and zebrafish offers powerful research capabilities.

Conclusions:

  • Recombinase-based techniques significantly enhance the utility of transgenesis in zebrafish.
  • These tools are vital for dissecting complex biological processes and modeling diseases.
  • Future developments promise even greater precision and application in zebrafish research.