Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Solvents01:12

Solvents

71.4K
A solvent is a substance, most often a liquid, that can dissolve other substances. Here, the substance being dissolved is called a solute. When a solvent and a solute combine, they form a solution - a homogenous mixture of both the solvent and the solute. Water is a universal biological solvent. Its polar structure allows it to dissolve many other polar compounds. The ability of water to dissolve is governed by a balance between water molecules binding to each other and binding to the solute.
A...
71.4K
Protein Transport to the Thylakoids01:22

Protein Transport to the Thylakoids

3.0K
Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phytochemical-Loaded Biodegradable Nanoemulsions for Eradication of Fungal Biofilms.

Nanomaterials (Basel, Switzerland)·2026
Same author

A Sensitive Multichannel Fluorescent Polymer Sensor Array for the Detection of Protein Fluctuations in Serum.

Sensors (Basel, Switzerland)·2026
Same author

Introduction to "Endocytosis and cellular delivery".

RSC chemical biology·2026
Same author

Incorporation of Functional Proteins on Cellular Surfaces via Artificial Cell-Derived Vesicles (ACDVs) for Plasma Membrane Reprogramming.

Journal of the American Chemical Society·2026
Same author

Controlled intra- and extracellular localization of bioorthogonal polymeric nanozymes.

Chemical science·2026
Same author

Polymeric Lysosome-Targeting Chimeras (PolyTACs): Extracellular Targeted Protein Degradation without Co-Opting Lysosome-Targeting Receptors.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Feb 14, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.4K

Supramolecular Assemblies for Transporting Proteins Across an Immiscible Solvent Interface.

Jingjing Gao1, Bo Zhao1, Meizhe Wang1

  • 1Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.

Journal of the American Chemical Society
|February 13, 2018
PubMed
Summary

Researchers developed polymeric supramolecular assemblies for protein transport across solvent interfaces. This method uses electrostatics and ligand-protein interactions, preserving protein structure and function for applications in sensing and catalysis.

More Related Videos

Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

10.5K
Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

19.3K

Related Experiment Videos

Last Updated: Feb 14, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

6.4K
Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

10.5K
Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

19.3K

Area of Science:

  • Supramolecular chemistry
  • Biotechnology
  • Materials science

Background:

  • Transporting proteins across incompatible solvent interfaces (e.g., aqueous to organic) is challenging.
  • Maintaining protein structure and function during interfacial transfer is crucial for applications.

Purpose of the Study:

  • To develop polymeric supramolecular assemblies capable of efficient protein transport across immiscible solvent phases.
  • To demonstrate the use of electrostatic and ligand-protein interactions for selective protein translocation.
  • To confirm the structural integrity and functional activity of transported proteins.

Main Methods:

  • Design and synthesis of novel polymeric supramolecular assemblies.
  • Utilizing electrostatic forces and specific ligand-protein binding for selective capture and transport.
  • Employing techniques to verify protein tertiary structure and biological function post-transport.

Main Results:

  • Successfully demonstrated protein transport from aqueous to organic phases using the developed assemblies.
  • Electrostatic and ligand-protein interactions were confirmed as key drivers for selective protein translocation.
  • Transported proteins retained their native tertiary structure and biological activity.

Conclusions:

  • Polymeric supramolecular assemblies offer an effective strategy for interfacial protein transport.
  • The approach preserves protein integrity, enabling new applications.
  • This work opens avenues for supramolecular assemblies in sensing, diagnostics, and catalysis.