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Updated: Feb 14, 2026

Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Published on: October 14, 2021
Cultivation of multiple genera of hydrogenotrophic methanogens from different environmental niches
Akshay Joshi1, Vikram Lanjekar1, Prashant K Dhakephalkar1
1Bioenergy Group, Agharkar Research Institute, Pune, India.
Abstract:
Six genera of hydrogenotrophic methanogens, namely Methanobrevibacter, Methanobacterium, Methanocorpusculum, Methanothermobacter, Methanoculleus, and Methanospirillum were cultivated from diverse environmental niches like rumen, feces, gut, and sediments using BY medium. We also report a putative novel genus and two novel species of methanogens isolated from termite, Indian star tortoise, and green iguana.
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