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Updated: Aug 4, 2026
Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Published on: April 4, 2011
[Modification of the viral immunoassay for determining ultramicro-quantities of antigens]
A technique for ultramicro-quantitation of antigens is suggested. It is based on the antigen isolation by immunosorption and a subsequent assay using phage-antigen conjugate (modification of viral immunoassay). Antibody Fab' fragments were bound to both the immunosorbent and the conjugate through their terminal thio groups. The technique sensitivity was one-two orders higher than that of ELISA for the corresponding antigens.
A technique for ultramicro-quantitation of antigens is suggested. It is based on the antigen isolation by immunosorption and a subsequent assay using phage-antigen conjugate (modification of viral immunoassay). Antibody Fab' fragments were bound to both the immunosorbent and the conjugate through their terminal thio groups. The technique sensitivity was one-two orders higher than that of ELISA for the corresponding antigens.
