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Fas-associated protein factor 1 is involved in meiotic resumption in mouse oocytes

Hui Peng1, Jianchao Huo1, Yuyun Gao1

  • 1College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P. R. China.

The Journal of Reproduction and Development
|February 14, 2018
PubMed

Insights

Fas-associated protein factor 1 (FAF1) plays a crucial role in mouse oocyte maturation. Knocking down FAF1 significantly impairs germinal vesicle breakdown, highlighting its novel function in meiotic resumption.

Area of Science:

  • Reproductive biology
  • Molecular and cellular biology
  • Developmental biology

Background:

  • Fas-associated protein factor 1 (FAF1) is involved in various cellular functions.
  • Mutations in Faf1 have been linked to embryonic lethality in mouse models.
  • The role of FAF1 in oocyte meiosis remains largely unexplored.

Purpose of the Study:

  • To investigate the expression pattern and localization of FAF1 in mouse oocytes.
  • To elucidate the function of FAF1 during meiotic resumption in mouse oocytes.

Main Methods:

  • Quantitative analysis of Faf1 mRNA and protein expression during oocyte maturation.
  • Immunofluorescence to determine FAF1 subcellular localization.
  • RNA interference (RNAi) to knockdown Faf1 expression in germinal vesicle (GV)-stage oocytes.
  • Assessment of meiotic resumption, specifically germinal vesicle breakdown (GVBD).

Main Results:

  • FAF1 is exclusively expressed in oocytes across different follicular stages and localized predominantly in the cytoplasm of growing oocytes.
  • Faf1 mRNA and protein are consistently present throughout oocyte maturation, with stable mRNA levels from GV to metaphase II (MII) stages.
  • Knockdown of Faf1 in GV-stage oocytes resulted in a significant reduction in the rate of GVBD.

Conclusions:

  • FAF1 is expressed and localized within mouse oocytes during maturation.
  • FAF1 plays a critical role in regulating meiotic resumption, specifically GVBD.
  • This study reveals a novel function for FAF1 in the meiotic process of mouse oocytes.
Keywords:
FAF1 proteinMeiotic resumptionMouseOocyte

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