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Updated: Feb 14, 2026

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
Published on: May 9, 2019
CellCycleTRACER accounts for cell cycle and volume in mass cytometry data
Maria Anna Rapsomaniki1, Xiao-Kang Lun2,3, Stefan Woerner1
1Zürich Research Lab, IBM, Säumerstrasse 4, 8803, Rüschlikon, Switzerland.
Abstract:
Recent studies have shown that cell cycle and cell volume are confounding factors when studying biological phenomena in single cells. Here we present a combined experimental and computational method, CellCycleTRACER, to account for these factors in mass cytometry data. CellCycleTRACER is applied to mass cytometry data collected on three different cell types during a TNFα stimulation time-course. CellCycleTRACER reveals signaling relationships and cell heterogeneity that were otherwise masked.
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