Related Experiment Video
Updated: Feb 14, 2026

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
Published on: February 26, 2018
Author Correction: Sister chromatid resolution is an intrinsic part of chromosome organization in prophase
Kota Nagasaka1,2, M Julius Hossain3, M Julia Roberti3
1Cancer Institute of the Japanese Foundation for Cancer Research, Division of Experimental Pathology, 3-8-31 Ariake, Koto-ku, Tokyo, 135-8550, Japan.
Abstract:
In the version of this Letter originally published, the authors omitted a citation of an early study demonstrating topoisomerase-II-dependent sister chromatid resolution. This reference has now been added to the reference list as reference number 28, and the relevant text has been amended as follows to include its citation: 'Resolution must reflect the removal of sister-sister contacts, and we show here that Topo-IIα-mediated release of DNA catenation plays a major role (Fig. 4), in agreement with previous findings28, whereas, surprisingly, cohesin dissociation is not strictly required (Fig. 3).' Subsequent references have been renumbered. All online versions of the Letter have been updated to reflect this change.
Related Concept Videos
Attachment of Sister Chromatids
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Lampbrush Chromosomes
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
Milgram's Obedience to Authority
Crossing Over
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...

