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Genistein inhibits the S-phase kinase-associated protein 2 expression in breast cancer cells

Dengfeng Ye1, Zhian Li1, Chunshou Wei1

  • 1Department of Oncological Surgery, The Second Hospital of Shaoxing, Shaoxing, Zhejiang 312000, P.R. China.

Experimental and Therapeutic Medicine
|February 14, 2018
PubMed

Insights

Genistein, a soy isoflavone, suppresses breast cancer growth by inhibiting S-phase kinase-associated protein 2 (Skp2). This action promotes tumor suppressor proteins p21 and p27, offering a potential new treatment strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Breast cancer is a leading cause of cancer-related deaths in women globally.
  • Genistein demonstrates tumor suppressor activity, but its precise mechanisms in breast cancer require further elucidation.
  • S-phase kinase-associated protein 2 (Skp2) is implicated in the progression of various human cancers.

Purpose of the Study:

  • To investigate if genistein inhibits breast cancer progression by suppressing Skp2.
  • To elucidate the molecular mechanisms underlying genistein's anti-tumor effects in breast cancer cells.

Main Methods:

  • Breast cancer cell lines were treated with genistein.
  • Cell proliferation, invasion, migration, apoptosis, and cell cycle were assessed.
  • Expression levels of Skp2, p21, and p27 were analyzed.

Main Results:

  • Genistein significantly inhibited breast cancer cell proliferation, invasion, and migration.
  • Genistein induced apoptosis and G2/M cell cycle arrest.
  • Genistein treatment downregulated Skp2 expression and upregulated p21 and p27 expression.

Conclusions:

  • Genistein exerts its tumor suppressor effects in breast cancer, at least partially, by inhibiting Skp2.
  • Genistein promotes the expression of downstream tumor suppressors, p21 and p27.
  • Targeting Skp2 with genistein presents a promising therapeutic avenue for breast cancer treatment.
Keywords:
S-phase kinase associated protein 2apoptosisbreast cancergenisteinproliferation

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