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PTX3 in serum induces renal mesangial cell proliferation but has no effect on apoptosis

Danhuan Zhang1, Minhui Xi2, Lingyun Chen1

  • 1Department of Nephrology, Tongren Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200336, P.R. China.

Experimental and Therapeutic Medicine
|February 14, 2018
PubMed

Insights

Silencing pentraxin 3 (PTX3) in human glomerular mesangial cells (HMCs) inhibits proliferation via MAPK pathways. This study found PTX3 knockdown reduced HMC viability but did not affect apoptosis.

Area of Science:

  • Nephrology
  • Cell Biology
  • Molecular Biology

Background:

  • Pentraxin 3 (PTX3) is a key component of the innate immune system.
  • Its role in regulating human glomerular mesangial cell (HMC) proliferation and apoptosis requires further investigation.

Purpose of the Study:

  • To investigate the effect of pentraxin 3 (PTX3) on the regulation of proliferation and apoptosis in human glomerular mesangial cells (HMCs).

Main Methods:

  • Small interfering (si)RNA was used to inhibit endogenous PTX3 expression in HMCs.
  • Cell proliferation was assessed using MTT assays, and apoptosis was analyzed by flow cytometry.
  • Western blot analysis was performed to detect mitogen-activated protein kinase (MAPK) pathway activation.

Main Results:

  • PTX3-siRNA significantly decreased PTX3 mRNA expression in HMCs.
  • HMC viability was significantly reduced following PTX3 knockdown.
  • PTX3 silencing led to decreased phosphorylation of p38 MAPK, ERK1/2, and JNK signaling pathways.
  • No significant effect on early and late apoptotic cell populations was observed.

Conclusions:

  • Silencing PTX3 inhibits HMC proliferation through the MAPK signaling pathways.
  • PTX3 does not appear to influence HMC apoptosis.
Keywords:
apoptosisglomerular mesangial cellspentraxin 3proliferation

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