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Updated: Feb 14, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid
Juliano Da Silveira1, Gabriella M Andrade1, Felipe Perecin1
1Faculty of Animal Sciences and Food Engineering, Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, SP, Brazil.
Abstract:
Mammalian ovarian follicular growth is characterized by development of a large fluid filled antrum that separates mural granulosa cells and cumulus cells. Extensive communication between the different cell types is necessary for maturation of a developmentally competent oocyte. Here, we describe an approach for the isolation of cell-secreted exosomes from ovarian follicular fluid, identification of small RNAs (i.e., microRNAs) in exosomes, labeling of exosomes, and examining cell uptake of exosomes by follicular cells.
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