Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prosomes exist in plant cells too.

A Kremp, M Schliephacke, U Kull

    Experimental Cell Research
    |October 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Validation of the standardization framework SSTR-RADS 1.0 for neuroendocrine tumors using the novel SSTR‑targeting peptide [<sup>18</sup>F]SiTATE.

    European radiology·2024
    Same author

    [Melanosis of the bladder-a rare diagnosis].

    Urologie (Heidelberg, Germany)·2023
    Same author

    Outcome groups and a practical tool to predict success of shock wave lithotripsy in daily clinical routine.

    World journal of urology·2020
    Same author

    Advanced non-contrasted computed tomography post-processing by CT-Calculometry (CT-CM) outperforms established predictors for the outcome of shock wave lithotripsy.

    World journal of urology·2018
    Same author

    Energy content, storage substances, and construction and maintenance costs of Mediterranean deciduous leaves.

    Oecologia·2017
    Same author

    Erectile function following brachytherapy, external beam radiotherapy, or radical prostatectomy in prostate cancer patients.

    Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2015

    Researchers purified tobacco 19S particles, finding they contain RNA and protein. These plant particles share structural and biochemical traits with animal prosomes.

    Area of Science:

    • Plant Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Prosomess are known as small cytoplasmic complexes in animal cells.
    • The presence and function of similar structures in plant cells remain largely unexplored.

    Purpose of the Study:

    • To investigate the biochemical and structural characteristics of 19S particles in tobacco (Nicotiana rustica) leaf cells.
    • To determine if these plant particles are analogous to animal prosomes.

    Main Methods:

    • Purification of 19S particles from tobacco leaf cells.
    • Density gradient centrifugation (Cs2SO4-DMSO) to determine particle composition.
    • Polyacrylamide gel electrophoresis (PAGE) for protein and RNA analysis.
    • Electron microscopy for structural examination.

    Related Experiment Videos

    Main Results:

    • Purified 19S particles exhibited a density of 1296 g/cm3, indicating RNA and protein content.
    • PAGE revealed eight distinct proteins (20-30 kD) and RNA (70-80 nucleotides).
    • Electron microscopy showed a raspberry-shaped structure with a central depression, similar to animal prosomes.

    Conclusions:

    • Tobacco 19S particles are small cytoplasmic complexes.
    • These plant particles possess biochemical and structural similarities to animal prosomes.
    • This suggests a conserved role for prosome-like structures across different eukaryotic kingdoms.