Related Experiment Video
Updated: Feb 14, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
Published on: December 4, 2010
The mRNA export factor Sac3 maintains nuclear homeostasis and regulates cytoskeleton organization in Candida albicans
Chenpeng Xiao1, Qilin Yu1, Bing Zhang1
1Key Laboratory of Molecular Microbiology & Technology, Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
Aim:
In eukaryotes, the nuclear export of mRNAs is essential for gene expression and regulations of numerous cellular processes. This study aimed to identify the mRNA export factor Sac3 in Candida albicans.
Materials & Methods:
A sac3Δ/Δ mutant was obtained using PCR-mediated homologous recombination.
Results:
Disruption of SAC3 caused abnormal accumulation of mRNA in the nuclei. Further investigations revealed that sac3Δ/Δ mutant exhibited a severely growth defect, which was related to abnormal aggregation of microtubules. Moreover, loss of Sac3 caused a defect in hyphal polarized growth, which was associated with depolarization of actin cytoskeleton. In addition, the virulence of sac3Δ/Δ mutant was seriously attenuated.
Conclusion:
Our findings provide new insights into the mRNA export factor Sac3 in C. albicans.
Related Concept Videos
Nuclear Export of mRNA
Nuclear Export of mRNA
Regulated mRNA Transport
Regulated mRNA Transport
Nuclear Export
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...

