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Updated: Feb 14, 2026
Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
Jiazhang Lian1,2, Mohammad HamediRad2, Huimin Zhao2,3
1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Abstract:
Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular hosts for industrial biotechnology. This review summarizes the recent development of this disruptive technology for metabolic engineering applications, including CRISPR-mediated gene knock-out and knock-in as well as transcriptional activation and interference. More importantly, multi-functional CRISPR systems that combine both gain- and loss-of-function modulations for combinatorial metabolic engineering are highlighted.
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