Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.3K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.3K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.7K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
Line Loss01:10

Line Loss

550
The different configurations of source-load connections include wye (star) and delta connections. The relationship between line and phase voltages and currents varies depending on the configuration. When the source is supplying power, it is transmitted through the wires to the load, and during this transmission, some power is absorbed by the wires, leading to line loss.
Line loss impacts power delivery efficiency in a balanced three-phase circuit. The symmetry in such a circuit simplifies the...
550
Anatomy of the Intestines01:23

Anatomy of the Intestines

88.0K
Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
88.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same author

ATR kinase inhibitors induce mitochondrial fission in CD8<sup>+</sup> T cells and impair immune memory <i>in vivo</i>.

bioRxiv : the preprint server for biology·2026
Same author

Transient ATR inhibition following ionizing radiation enhances immune-mediated antitumor response and survival.

bioRxiv : the preprint server for biology·2026
Same author

Pharmacokinetics of venetoclax in a patient after whipple pancreaticoduodenectomy.

Cancer chemotherapy and pharmacology·2026
Same author

Phase 2 study of cabozantinib (XL184) with nivolumab and ipilimumab for the treatment of poorly differentiated neuroendocrine carcinomas (ETCTN10315).

The oncologist·2026
Same author

A phase 1 study of berzosertib (M6620, VX-970) in combination with cisplatin and radiation in patients with locally advanced head and neck squamous cell carcinoma (ETCTN 9950).

Cancer·2026

Related Experiment Video

Updated: Feb 14, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K

Targeting p53-dependent stem cell loss for intestinal chemoprotection.

Brian J Leibowitz1,2, Liheng Yang2,3, Liang Wei1,2

  • 1Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

Science Translational Medicine
|February 14, 2018
PubMed
Summary
This summary is machine-generated.

Targeting PUMA protects against chemotherapy-induced intestinal injury by preserving LGR5+ stem cells. This strategy selectively blocks damage to normal cells, leaving anti-cancer effects intact.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

11.3K

Related Experiment Videos

Last Updated: Feb 14, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.8K
Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.1K
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

11.3K

Area of Science:

  • Gastroenterology
  • Oncology
  • Molecular Biology

Background:

  • The gastrointestinal (GI) epithelium, rapidly renewing tissue, is maintained by stem cells.
  • Chemotherapy and radiotherapy cause dose-limiting GI side effects, impacting cancer patient quality of life.
  • p53 is a critical DNA damage response regulator with cell-type-specific outcomes.

Purpose of the Study:

  • To investigate the role of p53-dependent PUMA induction in chemotherapy-induced intestinal injury.
  • To evaluate PUMA inhibition as a strategy for normal intestinal chemoprotection.

Main Methods:

  • Utilized mouse models with genetic ablation of Puma and p53.
  • Employed a small-molecule PUMA inhibitor (PUMAi) in vivo and in vitro.
  • Assessed LGR5+ stem cell loss, WNT/NOTCH signaling, and apoptosis in colonic organoids.

Main Results:

  • p53-dependent PUMA induction mediates chemotherapy-induced lethal GI injury in mice.
  • Genetic ablation of Puma, but not p53, protected against lethal GI injury.
  • PUMAi prevented stem cell loss, signaling pathway activation, and exhaustion during repeated chemotherapy.
  • PUMAi protected human and mouse colonic organoids from chemotherapy-induced damage without affecting cancer cells.

Conclusions:

  • Targeting PUMA is a promising strategy for normal intestinal chemoprotection.
  • Selective PUMA inhibition preserves p53-dependent stem cell function during chemotherapy.
  • This approach spares p53-dependent anti-cancer effects while protecting normal tissues.