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Related Experiment Videos

Dynamin's helical geometry does not destabilize membranes during fission.

Zachary A McDargh1, Markus Deserno2

  • 1Chemical Engineering Department, Columbia University, New York, New York.

Traffic (Copenhagen, Denmark)
|February 14, 2018
PubMed
Summary

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Dynamin-mediated membrane fission is mechanical. This study refutes a lipid bilayer instability model, supporting a new mechanism where dynamin drives membranes up an energy barrier for fission.

Area of Science:

  • Biophysics
  • Cell Biology
  • Molecular Motors

Background:

  • Dynamin mediates membrane fission via GTP-dependent conformational changes.
  • The precise mechanical link between dynamin's action and membrane scission remains elusive.
  • Previous models proposed geometric changes in dynamin's helix induce lipid bilayer instability.

Purpose of the Study:

  • To re-evaluate the proposed lipid bilayer instability model for dynamin-mediated fission.
  • To investigate the role of dynamin's helical geometry in membrane scission.
  • To explore alternative mechanisms for dynamin-driven membrane fission.

Main Methods:

  • Computational modeling of dynamin's helical structure and its interaction with lipid bilayers.
  • Analysis of mechanical forces and energy landscapes during membrane constriction.
Keywords:
Helfrich theorydifferential equationdynaminendocytosisgeometrymembrane fissionmembrane mechanicsmembrane shape equationmodelingvesicle scission

Related Experiment Videos

  • Comparison of simulation results with existing experimental data.

    • Main Results:

    • Dynamin's conformational changes do not induce a mechanical instability in the lipid bilayer.
    • The proposed soap film-like instability model for membrane fission is not supported.
    • An alternative model suggests dynamin elevates the membrane energy barrier.

    Conclusions:

    • Dynamin-mediated fission does not rely on inducing a lipid bilayer mechanical instability.
    • Dynamin likely functions by driving membranes up an energy barrier.
    • Thermal fluctuations then facilitate the transition to hemifission and subsequent scission.