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High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and

Liang-Xing Fang1,2, Xing-Ping Li1,2, Guo-Hui Deng1,2

  • 1National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, People's Republic of China.

Antimicrobial Agents and Chemotherapy
|February 14, 2018
PubMed

Insights

A novel fusion plasmid, pP2-3T, combines multidrug resistance (MDR) and virulence genes. This IncHI2 plasmid lineage shows genetic plasticity, acquiring resistance genes and fusing with other plasmids, necessitating surveillance in Enterobacteriaceae.

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Multidrug resistance (MDR) in bacteria is a growing public health concern.
  • Plasmids are key vectors for the spread of antibiotic resistance genes (ARGs).
  • IncHI2 plasmids are increasingly recognized for their role in disseminating MDR.

Purpose of the Study:

  • To characterize a novel fusion plasmid, pP2-3T, mediating MDR and virulence.
  • To investigate the phylogenetic and genomic features of ST3-IncHI2 plasmids.
  • To understand the genetic plasticity and evolution of MDR IncHI2 plasmids.

Main Methods:

  • Plasmid isolation and characterization.
  • Phylogenetic analysis using whole-genome sequencing data.
  • Comparative genomics to identify mobile genetic elements and ARGs.

Main Results:

  • A novel fusion plasmid, pP2-3T, was identified, integrating ST3 with an IncFII backbone, conferring MDR and virulence.
  • Phylogenetic analysis revealed a distinct IncHI2 lineage characterized by conserved backbones and high genetic plasticity.
  • These plasmids have acquired numerous ARGs and fused with other plasmids, contributing to their adaptability.

Conclusions:

  • The pP2-3T plasmid represents a significant fusion event contributing to MDR and virulence.
  • The identified IncHI2 lineage demonstrates remarkable genetic plasticity, facilitating ARG acquisition and dissemination.
  • Enhanced surveillance for multiresistance IncHI2 plasmids in Enterobacteriaceae is crucial to monitor and control their spread.
Keywords:
IncHI2 plasmidscointegrated plasmidscomparative genomicsevolutionmultidrug resistance

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