Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Homeostasis?01:16

What is Homeostasis?

57.5K
Maintaining homeostasis requires that the body continuously maintain its internal conditions. Each physiological condition has a particular set point, from body temperature to blood pressure to levels of certain nutrients. A set point is the physiological value around which the normal range fluctuates. A normal range is a restricted set of values that is optimally healthful and stable. For example, the set point for normal human body temperature is approximately 37°C (98.6°F).
57.5K
pH Homeostasis01:31

pH Homeostasis

20.1K
Acid-base homeostasis is essential for maintaining normal physiological activities in humans. The pH of various body fluids is strictly regulated because it is critical for the optimal activity of enzymes involved in metabolic reactions. Enzymes are basically proteins, so, any significant change in pH can affect their structure and activity. In humans, pH is regulated using three primary mechanisms— chemical buffer systems, respiratory regulation, and renal regulation.
Respiratory...
20.1K
Phosphate Buffer01:22

Phosphate Buffer

5.4K
The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
5.4K
Skeleton and Calcium Homeostasis01:21

Skeleton and Calcium Homeostasis

6.1K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
6.1K
Synthesis and Decomposition Reactions02:17

Synthesis and Decomposition Reactions

38.4K
Synthesis and decomposition are two types of redox reactions. Synthesis means to make something, whereas decomposition means to break something. The reactions are accompanied by chemical and energy changes. 
38.4K
Dehydration Synthesis01:15

Dehydration Synthesis

150.6K
Overview
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
150.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short autoinhibitory sequences control phase separation of an essential bacterial transcription termination factor.

The EMBO journal·2026
Same author

A conserved peptidase governs glucose homeostasis in <i>Bacteroides</i>.

bioRxiv : the preprint server for biology·2026
Same author

The master virulence regulator PhoP dictates carbon metabolism by controlling cyclic AMP synthesis in Salmonella.

PLoS biology·2025
Same author

How Pathogens Maintain Proteostasis During Infection.

Molecular microbiology·2025
Same author

Hierarchical glycolytic pathways control the carbohydrate utilization regulator in human gut Bacteroides.

Nature communications·2025
Same author

Infection-relevant conditions dictate differential versus coordinate expression of <i>Salmonella</i> chaperones and cochaperones.

mBio·2025

Related Experiment Video

Updated: Feb 14, 2026

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals
08:54

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals

Published on: May 25, 2016

8.9K

Protein synthesis controls phosphate homeostasis.

Mauricio H Pontes1,2, Eduardo A Groisman1,2

  • 1Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, Connecticut 06536, USA.

Genes & Development
|February 14, 2018
PubMed
Summary
This summary is machine-generated.

Impaired protein synthesis triggers a phosphate (Pi) starvation response, even with sufficient external Pi. This occurs because reduced protein synthesis lowers cellular adenosine triphosphate (ATP) levels, mimicking Pi scarcity.

Keywords:
ATPMg2+PhoB/PhoRPhoP/PhoQPitranslation

More Related Videos

Revealing Electromechanical Control of Tissue Homeostasis Using a Two-Layer Microfluidic Device
11:08

Revealing Electromechanical Control of Tissue Homeostasis Using a Two-Layer Microfluidic Device

Published on: September 19, 2025

1.2K
Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
06:27

Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans

Published on: September 12, 2020

5.7K

Related Experiment Videos

Last Updated: Feb 14, 2026

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals
08:54

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals

Published on: May 25, 2016

8.9K
Revealing Electromechanical Control of Tissue Homeostasis Using a Two-Layer Microfluidic Device
11:08

Revealing Electromechanical Control of Tissue Homeostasis Using a Two-Layer Microfluidic Device

Published on: September 19, 2025

1.2K
Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans
06:27

Assessment of de novo Protein Synthesis Rates in Caenorhabditis elegans

Published on: September 12, 2020

5.7K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Cellular Physiology

Background:

  • Orthophosphate (Pi) is vital for cellular functions and is typically imported when scarce.
  • Cells possess mechanisms to sense and respond to Pi availability in their environment.

Purpose of the Study:

  • To investigate the link between protein synthesis and the cellular phosphate (Pi) starvation response.
  • To determine if impaired protein synthesis can trigger Pi homeostasis mechanisms.

Main Methods:

  • Studied the bacterium *Salmonella enterica* serovar Typhimurium.
  • Investigated the role of magnesium (Mg2+) in protein synthesis and Pi homeostasis.
  • Examined the effects of pharmacological inhibition of protein synthesis in *Escherichia coli* and *Saccharomyces cerevisiae*.

Main Results:

  • Impaired protein synthesis, independent of external Pi levels, activates the Pi starvation response in *Salmonella*.
  • This response involves PhoB protein phosphorylation and upregulation of Pi transporter genes.
  • The response is transient and linked to fluctuations in cytoplasmic magnesium (Mg2+) and adenosine triphosphate (ATP) levels.
  • Similar responses were observed in *Escherichia coli* and *Saccharomyces cerevisiae* upon protein synthesis inhibition.

Conclusions:

  • Protein synthesis directly influences cellular phosphate (Pi) homeostasis.
  • A conserved regulatory link exists between protein synthesis and Pi regulation across different organisms.
  • This finding reveals a novel mechanism controlling Pi uptake and utilization.