Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Size

Cell Size

Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diverse infection models demonstrate robust resistance of <i>Mycobacterium tuberculosis</i> to innate immunity.

bioRxiv : the preprint server for biology·2026

Same author

NLRP1B is an integrated decoy that subverts <i><i>Shigella</i> flexneri</i> E3 ligase activity to promote effector-triggered immunity.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Poxvirus attack of antiviral defense pathways unleashes an effector-triggered NF-κB response.

Science (New York, N.Y.)·2026

Same author

Strong sustained type I IFN signaling acts cell intrinsically to impair IFNγ responses and cause tuberculosis susceptibility.

bioRxiv : the preprint server for biology·2026

Same author

<i>Shigella</i> OspF blocks rapid p38-dependent priming of the NAIP-NLRC4 inflammasome.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Tuberculosis susceptibility in genetically diverse mice reveals functional diversity of neutrophils.

eLife·2025

Same journal

Retraction: In vivo NCL targeting affects breast cancer aggressiveness through miRNA regulation.

The Journal of experimental medicine·2026

Same journal

Intravesical mesothelin-based CAR T cells targeting MUC16 effectively control bladder cancer in preclinical models.

The Journal of experimental medicine·2026

Same journal

Flawed translation triggers oncogenic B-T cell communication.

The Journal of experimental medicine·2026

Same journal

Correction: LCK'ed in: Inborn errors of immunity in LCK reveal how TCR signaling is calibrated.

The Journal of experimental medicine·2026

Same journal

Mechanobiology of inflammation: Pulling the strings of innate immunity.

The Journal of experimental medicine·2026

Same journal

Bile acid retention in efferocytic macrophages shapes their inflammatory status during cholangitis.

The Journal of experimental medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 14, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

Defusing inflammasomes.

Andrew Sandstrom¹, Russell E Vance²

¹Howard Hughes Medical Institute and Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA sandstrom@berkeley.edu.

The Journal of Experimental Medicine

|February 15, 2018

Summary

This summary is machine-generated.

Researchers isolated active caspase-1 from macrophages following inflammasome activation. They discovered that caspase-1 rapidly becomes inactive due to self-processing, a key finding in inflammatory response research.

More Related Videos

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Related Experiment Videos

Last Updated: Feb 14, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Area of Science:

Immunology
Molecular Biology
Cellular Signaling

Background:

The inflammasome is a multiprotein complex crucial for innate immunity.
Caspase-1 activation is central to inflammatory processes and cytokine maturation.
Understanding caspase-1 regulation is vital for controlling inflammatory diseases.

Purpose of the Study:

To investigate the activity and regulation of caspase-1 after inflammasome activation in macrophages.
To elucidate the post-translational modifications and inactivation mechanisms of caspase-1.

Main Methods:

Isolation of active caspase-1 from murine bone marrow-derived macrophages (BMDMs).
Stimulation of inflammasome activation using nigericin.
Biochemical assays to assess caspase-1 activity and autoproteolytic processing.

Main Results:

Active caspase-1 was successfully isolated from macrophages post-inflammasome activation.
A surprising finding was the rapid autoinactivation of caspase-1.
Autoproteolytic processing was identified as the mechanism leading to caspase-1 inactivation.

Conclusions:

Caspase-1 activity is transient, being rapidly downregulated by autoproteolysis.
This autoinactivation mechanism represents a critical regulatory checkpoint for inflammasome signaling.
The findings provide new insights into the control of inflammatory responses mediated by caspase-1.