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Related Experiment Videos

IgD-binding factors from mouse T lymphocytes.

M Adachi, K Ishizaka

    Proceedings of the National Academy of Sciences of the United States of America
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    Normal mouse splenic lymphocytes produce T-cell factors that inhibit IgD rosette formation. These IgD-binding factors, derived from T cells with Fc delta receptors, are induced by IgD binding.

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Immunoglobulin D (IgD) plays a crucial role in B-cell development and immune responses.
    • T cells expressing Fc receptors for immunoglobulins can modulate immune cell interactions.
    • Understanding T-cell factor production is key to deciphering immune regulation.

    Purpose of the Study:

    • To investigate the formation and function of T-cell factors induced by dimeric mouse IgD.
    • To characterize the nature and source of these IgD-induced T-cell factors.
    • To determine if these factors selectively inhibit IgD receptor-mediated cell interactions.

    Main Methods:

    • Incubation of mouse splenic lymphocytes with dimeric mouse IgD.
    • Assay for rosette formation inhibition using IgD-coated erythrocytes.

    Related Experiment Videos

  • Isolation and characterization of rosette-inhibiting factors using IgD-Sepharose chromatography.
  • Determination of molecular weights of the factors via gel filtration or SDS-PAGE.
  • Identification of the T-cell subset source via cell surface marker analysis (Lyt-1).

    • Main Results:

    • Dimeric IgD induced T-cell factors that selectively inhibit rosette formation of Fc delta receptor-positive lymphocytes.
    • The inhibitory factors were identified as IgD-binding factors, binding to IgD-Sepharose and eluting at acidic pH.
    • IgE-binding factors and IgG-binding factors did not inhibit IgD rosettes, confirming specificity.
    • The IgD-binding factors comprised two species with molecular weights of 78,000 and 37,000.
    • These factors originated from Lyt-1+ T cells expressing Fc delta receptors.

    Conclusions:

    • Binding of IgD to Fc delta receptors on specific T cells induces the formation of IgD-binding factors.
    • These T-cell-derived IgD-binding factors play a role in regulating IgD receptor-mediated lymphocyte interactions.
    • The findings elucidate a novel mechanism of immune regulation involving T-cell-derived factors specific for IgD.