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MiR-139 prompts the development of osteosarcomas mainly through targeting ROCK1

Shaojun Su, Xinguang Nie

    Die Pharmazie
    |February 15, 2018
    PubMed

    Insights

    MicroRNA-139 (miR-139) acts as a tumor suppressor in osteosarcoma (OS) by inhibiting ROCK1. This study reveals miR-139

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • Aberrant microRNA (miRNA) expression is implicated in various cancers.
    • The role of miR-139 in osteosarcoma (OS) development and progression remains unexplored.

    Purpose of the Study:

    • To investigate the function of miR-139 in osteosarcoma.
    • To identify the molecular targets of miR-139 in OS cells.

    Main Methods:

    • Quantitative real-time PCR to measure miR-139 and ROCK1 expression.
    • Cell viability and invasion assays to assess miR-139 function.
    • Dual-luciferase reporter assays to confirm ROCK1 as a direct target of miR-139.

    Main Results:

    • miR-139 expression was significantly downregulated in OS tissues and cell lines.
    • ROCK1 expression was markedly upregulated in OS tissues.
    • Overexpression of miR-139 suppressed OS cell proliferation and invasion.
    • ROCK1 was validated as a direct target of miR-139.
    • ROCK1 overexpression reversed the anti-invasive effects of miR-139.

    Conclusions:

    • miR-139 functions as a tumor suppressor in osteosarcoma.
    • The anti-tumor effects of miR-139 in OS are primarily mediated through the inhibition of ROCK1.

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