Related Experiment Video

Updated: Feb 14, 2026

On-Chip Endothelial Inflammatory Phenotyping
12:43

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

11.4K

MicroRNA-146 regulates the inflammatory cytokines expression in vascular endothelial cells during sepsis

Ning Gao, Lei Dong

    Die Pharmazie
    |February 15, 2018
    PubMed
    Abstract

    Insights

    MicroRNA-146 (miR-146) is downregulated in sepsis and suppresses inflammatory cytokines. Restoring miR-146 levels reduces sepsis-related inflammation and nuclear factor kappa B (NF-κB) activity.

    Area of Science:

    • Molecular Biology
    • Immunology
    • Sepsis Pathogenesis

    Background:

    • Sepsis is a life-threatening condition characterized by dysregulated host response to infection.
    • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and play roles in various biological processes, including inflammation.
    • The specific role of miR-146 in sepsis pathogenesis requires further elucidation.

    Purpose of the Study:

    • To investigate the functional role of microRNA-146 (miR-146) in sepsis.
    • To elucidate the underlying molecular mechanisms by which miR-146 influences sepsis development.

    Main Methods:

    • Human vascular endothelial cells (EA.hy926) were stimulated with lipopolysaccharide (LPS) and transfected with miR-146 mimics or inhibitors.
    • Expression levels of miR-146, inflammatory cytokines (TNF-α, IL-6, ICAM-1, E-selectin), and NF-κB activity were analyzed.

    Main Results:

    • LPS stimulation significantly downregulated miR-146 expression.
    • miR-146 mimics increased miR-146 expression, while inhibitors decreased it, in LPS-treated cells.
    • miR-146 mimics suppressed the LPS-induced upregulation of inflammatory cytokines and NF-κB activity, whereas miR-146 inhibitors exacerbated these effects.

    Conclusions:

    • MiR-146 plays a crucial role in sepsis pathogenesis.
    • MiR-146 suppresses the expression of inflammatory cytokines, potentially by inhibiting NF-κB signaling.
    • Modulating miR-146 levels may represent a therapeutic strategy for sepsis.

    More Related Videos

    Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response12:50

    Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

    Published on: September 15, 2017

    7.0K
    Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells16:24

    Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

    Published on: February 21, 2014

    20.7K

    Related Concept Videos

    Regulation of Expression Occurs at Multiple Steps02:24

    Regulation of Expression Occurs at Multiple Steps

    Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
    Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
    26.6K
    Regulation of Expression Occurs at Multiple Steps02:24

    Regulation of Expression Occurs at Multiple Steps

    4.0K
    MicroRNAs01:22

    MicroRNAs

    MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
    24.3K
    MicroRNAs01:22

    MicroRNAs

    MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
    4.1K
    Inflammatory Response I: Vascular and Cellular01:30

    Inflammatory Response I: Vascular and Cellular

    The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
    16.9K
    Regulation of Expression at Multiple Steps01:23

    Regulation of Expression at Multiple Steps

    The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
    1.4K