Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phosphofructokinase in normal thyroid tissue and thyroid neoplasms.

M C van der Heijden, J N Verhagen, G Rijksen

    Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
    |January 1, 1986
    PubMed
    Summary

    Phosphofructokinase (PFK) activity is significantly elevated in human thyroid carcinomas compared to normal tissue. Adenomas with higher proliferative activity also show increased PFK levels, suggesting its role in thyroid tumor development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Diagnosis and treatment of soft tissue tumours: the dutch nationwide-accepted consensus.

    Sarcoma·2008
    Same author

    [From gene to disease; hereditary non-spherocytic hemolytic anemia caused by pyruvate kinase deficiency].

    Nederlands tijdschrift voor geneeskunde·2002
    Same author

    Polo-like kinase-1 is a target of the DNA damage checkpoint.

    Nature cell biology·2000
    Same author

    p21 inhibits Thr161 phosphorylation of Cdc2 to enforce the G2 DNA damage checkpoint.

    The Journal of biological chemistry·2000
    Same author

    Negative growth regulation of SK-N-MC cells by bFGF defines a growth factor-sensitive point in G2.

    The Journal of biological chemistry·2000
    Same author

    Cyclin D3 regulates proliferation and apoptosis of leukemic T cell lines.

    The Journal of biological chemistry·1999

    Area of Science:

    • Biochemistry
    • Oncology
    • Enzymology

    Background:

    • Phosphofructokinase (PFK) is a key regulatory enzyme in glycolysis.
    • Thyroid carcinomas and adenomas are common endocrine neoplasms.
    • Understanding enzyme activity in tumors can reveal metabolic alterations.

    Purpose of the Study:

    • To investigate phosphofructokinase (PFK) activity and isozyme composition in human thyroid carcinomas, follicular adenomas, and normal thyroid tissue.
    • To correlate PFK activity with tumor proliferative activity and histopathological type.

    Main Methods:

    • Enzyme-linked assays were used to measure specific phosphofructokinase (PFK) activity.
    • Immunoprecipitation with anti-M antibodies was employed to assess PFK isozyme composition.
    • Histopathological criteria were used to evaluate tumor proliferative activity.

    Related Experiment Videos

    Main Results:

    • Specific PFK activity was significantly higher in thyroid carcinomas (0.129 +/- 0.070) than in normal thyroid tissue (0.028 +/- 0.009) (p < 0.001).
    • Follicular adenomas exhibited heterogeneous PFK activities, with the highest levels observed in tumors showing increased proliferative activity.
    • Papillary carcinomas displayed altered PFK isozyme profiles, with a reduced M-type and an increased P-type isoenzyme compared to follicular and undifferentiated carcinomas.

    Conclusions:

    • Elevated phosphofructokinase (PFK) activity is a hallmark of human thyroid carcinomas and highly proliferative adenomas.
    • PFK isozyme composition varies among thyroid tumor types, particularly in papillary carcinomas.
    • These findings suggest a role for altered PFK activity and isozyme shifts in thyroid tumorigenesis.